primer

A primer is a short single-stranded nucleic acid used by all living organisms in the initiation of DNA synthesis. DNA polymerase (responsible for DNA replication) enzymes are only capable of adding nucleotides to the 3’-end of an existing nucleic acid, requiring a primer be bound to the template before DNA polymerase can begin a complementary strand. Living organisms use solely RNA primers, while laboratory techniques in biochemistry and molecular biology that require in vitro DNA synthesis (such as DNA sequencing and polymerase chain reaction) usually use DNA primers, since they are more temperature stable.

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  1. B

    SBR primer in bathroom before tiling?

    Should I prime bare plaster in the bathroom with SBR before tiling? I've heard it will help with the water resistance and help the tile adhesion. The tile sizes are 600x300. Any help would be appreciated, Cheers Brian.
    • Brihutchie
    • Thread
    • bathroom canada plaster primer sbr tiling
    • Replies: 3
    • Forum: Canada
  2. E

    primer on backer board

    hi all going to use cement backer boards my question is do i primer both sides? im fixing using tlie adesive and screws to wall thanks george
  3. Sean-tries-to-tile

    Anyone know how to tile on seraphite? Primer?

    I have just been told this is seraphite, just about to tile it, wasn't going to prime as I thought it was just sand/cement. House is 1955. Cheers
  4. Sean-tries-to-tile

    Adhesive primer? and toilet box backerboard joinline

    Hi guys, I would be very grateful for some advice about wether it is necessary to use acrylic primer on the walls in my cloakroom toilet wall. I ask because to me it looks grey like sand/cement mix? Its a 1955 council house. The walls aren't smooth like normal plaster elsewhere in the house...
  5. T

    Primer before tiling

    Hello. New here so go easy please lol... I’ve cut my tiles to size and laid Mapei 1210 self leveller. I read about priming the slc before I tile so I bought the nx sbr primer from wickes and applied it neat. It skinned and I had to get the whole lot up. I’ve done it again with a 4:1 ratio and...
  6. M

    Which Tilemaster Primer should I use

    Should I use Tilemaster Prime Plus or Tilemaster Prime + Grip on plywood overboarding in preparation for tiling? Thanks.
    • milleniumaire
    • Thread
    • canada primer should tilemaster which
    • Replies: 2
    • Forum: Canada
  7. A

    My 1st Job HELP Painting Tiles

    Hi! We've decided to give our toilet/bathroom/laundry a soft and cheap renovation by painting some really BLUE floor tiles. We went to Bunnings and purchased the primer and also the top coat/colour of choice. I scrubbed the floors and sanded then, vacuumed and then placed a coat of the primer...
  8. P

    Primer before waterproofing?

    Good evening. I’ve got plasterboard walls. I’m going to use either bal primer apd or sbr and bal flex fibre plus. I’ve read that I have to waterproof the plaster board as well. In what order do I put the primer and waterproofing on? Which comes first? thank you for reading and helping
  9. B

    Removing Primer from walls/tiles/acrylic

    Hi chaps, I’m mid way through my first DIY tiling, taking 3 half tiled walls above a bath to full tile, then adding a shower. I stripped the paint off of the plasterboard where I wanted to add tiles, then primed it using Mapei plaster primer. Unfortunately despite using masking tape to mask...
  10. T

    Adhesive choice & primer

    Dear Tilers I'am tiling to 6mm no more ply ( pre primed ) overlayed on chipboard, floor is solid and reasonably even, joists @ 400mm centers. Area of 3.38m2 it is not a wet room & there is no shower. The tiles are 200x900x9 porcelain wood effect. I have selected the following adhesives...
  11. D

    Advice please!

    Had a builder in to build a garage, was planning on just painting garage base but was so uneven he laid self levelling compound. I could not paint so decided best was to tile myself with porcelain. went out to prep floor and sweep up and noticed the floor was cracked and hollow sounding and...
    • Doyley91
    • Thread
    • advice canada please primer self leveling compound
    • Replies: 3
    • Forum: Canada
  12. F

    A few questions before laying tiles

    Hi all, this is my first post on here although in attempting to Google answers to my questions I have come across several threads on this forum already which were really helpful. So now I thought it'd help to get advice specific to my situation...so here goes. These are all in relation to an...
    • freddyq
    • Thread
    • canada ditra drying glue laying primer questions screed tile tiles
    • Replies: 19
    • Forum: Canada
  13. B

    Correct dilution for MApei Primer G on cement render?

    What is the correct dilution for primer G on cement render? (the render has some SBR already mixed in and has totally cured/ dried) The Mapei pdf does not give an exact figure but says 1:1 to 1:3 depending on absorbency. Preumably the more absorbent the more you dilute? How can I tell if it...
  14. H

    Mapei primer G skinned over

    Hi, I've previously posted in this forum about my first failed tiling attempt. I was able to take up the tiles easily after they'd set and someone suggested my primer may have skinned over. Since then I've taken up all the adhesive and primer and have primed again with mapei primer g 1:1 and...
  15. R

    Nomoreply primer when tanking

    Hi there, I’ve seen the thread about backer boards water proof vs water resistant etc. We were going to use aquapanel and tank with ever build aqua seal tanking kit which comes with primer etc. However, the merchant had nomoreply instead. I’ve checked this and they seem to require us to prime...
  16. Mapei UK Marketing

    Tilers Forum Members Only - Mapei Ceramic Training Course

    Our ceramic products training course, held at the brand new Mapei Academy training facility and run by our award winning training team, will give you an overview of the products and services offered by Mapei UK. It will also give you the opportunity to see behind the scenes taking you on a...
    • Mapei UK Marketing
    • Resource
    • ceramic grout mapei mapei adhesives porcelain primer training waterproofing
    • Category: Training
  17. E

    Mapei Mapei renovation screed primer

    Hey guys I've got a new, tampered concrete floor. Not sure if I need primer before applying Mapei Renovation Screed If yes, I've got a ton of SBR. Will this work? The screed will be containing UFH pipes. Thanks ES
  18. N

    Rocatex acrylic primer with Rapid Level 30?

    Hi Has anyone got any experience of using Rocatex acrylic primer with Rapid Level 30? I’ve got a load of primer left as well as some RL30. Thanks Andy
    • Northers
    • Thread
    • canada level primer rapid
    • Replies: 2
    • Forum: Canada
  19. D

    Length of time between applying primer and fixing tiles

    I'm having some tiles taken up and redone as they were previously fixed incorrectly (mainly lack of adhesive coverage). The contractors are asking to return on two separate days first to take up the tiles and prime the screed, and the second day to fix the the tiles. My question is, should they...
    • DazedConfused
    • Thread
    • canada fixing primer tile tiles time
    • Replies: 44
    • Forum: Canada
  20. kiteboy

    Primer now or just before tiling

    Hi all Hopefully have a tiler comming this Wednesday to make a start on kitchen tiling Was going to prime today onto a gypsum screed with Bal APD but not sure if I should wait to just before tiling or not Was going to do a 50:50 first coat today then maybe wait until the day before starting...
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