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primer

A primer is a short single-stranded nucleic acid used by all living organisms in the initiation of DNA synthesis. DNA polymerase (responsible for DNA replication) enzymes are only capable of adding nucleotides to the 3’-end of an existing nucleic acid, requiring a primer be bound to the template before DNA polymerase can begin a complementary strand. Living organisms use solely RNA primers, while laboratory techniques in biochemistry and molecular biology that require in vitro DNA synthesis (such as DNA sequencing and polymerase chain reaction) usually use DNA primers, since they are more temperature stable.

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  1. J

    T&G boards tiling on top of????

    hello, ive got a job coming up and there could be a deal with the step at the entrance of the wet room. ive looked in tilemaster product guide and it says you can tile on top t&g boards after priming with prime+grip primer. Has anyone done this before??
  2. S

    Mapei primer G required?

    Please can anyone advise if Mapei primer G is required when using Mapei buildfix wall tile adhesive on newly (8 weeks ago) skimmed bathroom walls? Or can I just use the adhesive straight onto the plastered walls? Thanks very much for any advice
  3. Mapei UK Marketing

    Mapei Shower Waterproofing Kit - Installation Video

    An easy to use kit containing everything required to waterproof a shower in one tub. Contains: Mapegum WPS, Shower Waterproofing Kit Primer and Waterproofing tape. For further information on the Shower Waterproofing Kit visit products - http://bit.ly/2e6QU0R
  4. O

    Tiling onto ply

    Hi guys, OK, I know you shouldn't tile onto ply if avoided, in bathrooms especially, but.. Got a job to do a small amount of tiling behind the end of a 'free standing' bath and a sink/toilet below window height. The builders/plumber that prepped the area used ply to box the area. I am going to...
  5. Mapei UK Marketing

    Mapei Ceramic Product Training.

  6. Andy Allen

    Primer

    Do you use the same primer make as the adhesive or do you use say tilemaster primer and weber adhesive. I know manufacturers will say you have to use the same but there selling the stuff......in the real world is primer not just the same with different labels?
  7. U

    No primer

    talking to a rep ,he said no need to prime ply or plaster,the flexi addy already got built in polymers and addittives ,what you think
  8. C

    Primer help

    Hi guys, wonder if someone can help with wall primers! I am re tiling our shower over the bath, tanking the 2/3 of the walls. Due to last minute plumbing issues we have changed the wall in which the shower will be installed, however the walls have already been primed. :oops: So now i have the...
  9. C

    Tanking primer

    Hi guys, quick question. Is tanking primer that comes in the kits (everbuild aquaseal) suitable for priming walls prior to tiling that wont be tanked? I have alot of the stuff left id rather use up, was planning to use mapei plaster primer on remaining wall but its going alot furthur than i...
  10. D

    Priming Green Plasterboard

    Hello All Do you still need to prime Green plasterboard before tiling with a cement based adhesive & porcelain tiles 600mm x 300mm . I have primed the shower area & tanked with the Mapei Kit. Thanks all
  11. area tiling

    Ardex 51 Primer

    Anyone used this primer on a hemi hydrate liquid screed floor , says on the technical site only needs diluted 1 to 3 , and that one coat is sufficient!! Any primers I've used on these screeds before are all 2 coats rolled in opposite directions to each other ?
  12. J

    Bellitex Flow Screed

    Hi all I know I'm not a regular poster on here but would be grateful for some advice on a flow screed. My colleague is tiling a large floor that has been filled over the ufh with Bellitex floor screed made be Tarmac. The screeder has advised us that this Bellitex screed takes 21 days to cure...
  13. O

    Tanking Days Before Tiles Go On, Is It Ok?

    Bathroom will be tiled soon, probably upto a week from now. However to save time I dont mind tanking the over bath area myself. I have bought an aquaseal kit, in the various steps listed it says a minimum of 6 hours between first and second coat of tanking membrane. Hence time wasted around...
  14. C

    Marmox Multiboard - Priming

    Can anyone advise me on whether I should prime marmox multiboard with BAL SBR before tiling using BAL Single Part Flexible adhesive or can I tile straight onto the boards. Thanks
  15. C

    Tiling Over Plywood, Slightly Confused.com

    Hi I have to tile over plywood boxed in pipes and will be using Bal one part flexible adhesive. The instructions state for timber flooring you should overlay with exterior ply and prime all timber surfaces on back face and edges with neat BAL BOND SBR priming agent. So my question is why do you...
  16. B

    Mosaics On Wood

    Just a quick question,I have been asked to fit mosaics onto the front of a curved bar,the bar is veneer wood,I am after something to paint onto it before applying adhesive that will ensure the mosaics will stayed fixed,any ideas?
  17. D

    Tile Over Bitumen

    Any suggestions. I have removed parquet flooring in order to lay ceramic tiles. Questions are ... 1.Can I lay direct onto the old bitumen residue that is left on the concrete , no lumps left just thin residue. If so should I prime first with neoprene primer , then lay tiles. 2. Other area...
  18. S

    Confusion

    Hello, i posted this in adhesive forum yesterday but only got one reply so i thought i would try it here. Hello, Please forgive me if this information is already out there on these forums as i have searched but not found the exact combination of ingredients i have. So i have a small kitchen...
  19. S

    Going Round In Circles

    Hello, Please forgive me if this information is already out there on these forums as i have searched but not found the exact combination of ingredients i have. So i have a small kitchen around 2.5 sqm, i removed the previous tiles a couple of years ago, quite alot of them where cracked(i'm not...
  20. G

    Applying Mapei Primer G

    I've been told to apply primer G neat, then someone else said 50/50 with water depending on the type of surface it's going on to as you don't want a skin to form. Then, dilute depending on how it's absorbing. How do u guys apply, or doesn't it matter that much?
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