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The Manchester United defender suffered the injury on the Royal Bafokeng training pitches in the final minutes of the session.
Further to a scan arranged by the England medical staff immediately after training on Friday afternoon, Ferdinand has been ruled out for four-six weeks and will therefore miss the World Cup.
Tottenham Hotspur's Michael Dawson has been contacted today and will fly to South Africa on Friday evening.
Steven Gerrard will lead the England team as captain in the tournament.
England manager Fabio Capello said: "It is obviously bad news and everyone with the squad is very disappointed and sorry for Rio.
"It was an accidental injury in training, but had nothing to do with the pitch
ha ha, or if they don't they'll go down fighting, the other team, the ref, the opposing teams backroom staff. Anybody see this the other night, the argies losing it was one of the better bits BBC iPlayer - World Cup's Most Shocking Momentsdoubt it very much,i think the Argies will win it.