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Spacer DNA is a region of non-coding DNA between genes. The terms intergenic spacer (IGS) or non-transcribed spacer (NGS) are used particularly for the spacer DNA between the many tandemly repeated copies of the ribosomal RNA genes.In bacteria, spacer DNA sequences are only a few nucleotides long. In eukaryotes, they can be extensive and include repetitive DNA, comprising the majority of the DNA of the genome. In ribosomal DNA, there are spacers within and between gene clusters, called internal transcribed spacer (ITS) and external transcribed spacers (ETS), respectively. In animals, the mitochondrial DNA genes generally have very short spacers. In fungi, mitochondrial DNA spacers are common and variable in length, and they may also be mobile.Due to the non-coding nature of spacer DNA, its nucleotide sequence changes much more rapidly over time than nucleotide sequences coding for genes that are subject to selective forces. Although spacer DNA might not have a function that depends on its nucleotide sequence, it may have sequence-independent functions.Spacer DNA has practical applications that enable researchers and scientists to examine interactions between CRISPR proteins and bacteriophages.
Hi all,
Time for some pay back for all the advice.
Here is the DIY job I am doing. I have cleaned up the kitchen here ready to start. I have the gas man coming wed to connect the hob next week and I am in desperate need of a sink so the primary objective is to focus on getting at least the...
jgw1
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We decided after seeing a picture in a brochure to have full walls tiled around the bath, but just a backsplash over the basin.
Using bevelled edge brick tiles in half bond (right term?) the two walls worked out fine, but i've just realised i don't know how to finish the edge of backsplash...
dave180
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Just wondering what the best grout would be for a kitchen splashback. Would i need one with Microban or an epoxy grout? or will just a standard grout do?
Mfox
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Hi there, I'm renovating my kitchen on a budget and have just removed the tiles from behind the kitchen work surfaces and around the windows. My plan is to re tile it myself. The tiles were only four high. I was dreading the damage, but 90% of the tiles came off without a fight. There were a few...
Hi,
I'm looking for an experienced tiler for a kitchen job.
I have 4.25 sq mtr of floor which I would like laying with 400x400 12 mm limestone. I also have 2.5 sq mtr of splashback which I would like laying with 150x75 12mm travertine.
It's a big ask but I would like this work to take...
Beags
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hello all.got to start a 4m2 splashback in 100mm2 ceramics for a friend tomorrow and had a few questions.i havn`t seen the room yet but there is 1 window,3 double sockets,and the rest is just worktop to top unit.first off,he`s supplying all materials and has bought metal trim.i havn`t seen it...
boldydave
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Can someone tell me if the LASH clips sold on Tradetiler are just the same as the Tuscan levelling system? am i right in thinking that if you use these to pull up a tile level with an adjacent one then a void is left underneath the tile? or am i reading too much into it? anyone used them? please...
bgatty68
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Find yourself a uPVC window accessory outlet and buy a bag of mixed glass packers. Slivers of plastic varying from .5mm to 2mm in thickness colour coded. Superb aid for tiling when using tiles with varying sizes.
Clueless2
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Evening everyone...hope all are well. Just a quick one...has anyone used these lash clips that are on the market to eliminate lipage. I noticed Dave was selling them through tradetiler and watched the video. They seem a good idea...but a decent tiler shouldn't need them. Also noticed they...
Perfect Tiling
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Hi all,
Need to get some tile spacers for the following:
Entrance/kitchen FLOOR: 33sq/m of 10mm porcelain tile, tile size 300x600mm
Bathroom Floor: 5sq/m meters of 12mm porcelain tile, tile size 200x200mm
Bathroom wall (also used as shower backing) 20sq/m of 8.5mm 250x400mm.
Is there a norm...
any1 know where i can get some 2mm T spacers from asap? i have got 60m of 8x4's to do next week. did 55m last week of the same tile with normal 2mm spacers! i've looked everywhere on the net, can only find people out of stock! cheers!
has anyone one been useing the lash spacers for the large format porcelain tiles?
would like to know if they are worth the time and money, as i have about 100 metres of the stuff to put down.
have to say i like the idea no lipage.
thanks.
Hi all
What's the best spacer/gap size for large ceramic travertine effect wall tiles 300 x 450mm and floor 300 x 300mm, or is it persnal preferance I was going to go with 2mm.....???
Cheers.......:smilewinkgrin:
mmmadman
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hi all, i will soon be tiling my mates kitchen, well splashback in between worktop and wall units. will i get away with using tubbed addy for this job? the walls have been skimmed and the tiles are the little 4x4 inch buggers!
thanks
manghan
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Which sealer would you recommend for White Portuguese Semi-Polished Grained Limestone tiles for a kitchen spalshback?
In the kitchen I am also having a Florentina Silk Polished Marble tiled floor. I would imagine I should seal the floor as well.
Is it recommended to seal once before...
anit999
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