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spacer

Spacer DNA is a region of non-coding DNA between genes. The terms intergenic spacer (IGS) or non-transcribed spacer (NGS) are used particularly for the spacer DNA between the many tandemly repeated copies of the ribosomal RNA genes.In bacteria, spacer DNA sequences are only a few nucleotides long. In eukaryotes, they can be extensive and include repetitive DNA, comprising the majority of the DNA of the genome. In ribosomal DNA, there are spacers within and between gene clusters, called internal transcribed spacer (ITS) and external transcribed spacers (ETS), respectively. In animals, the mitochondrial DNA genes generally have very short spacers. In fungi, mitochondrial DNA spacers are common and variable in length, and they may also be mobile.Due to the non-coding nature of spacer DNA, its nucleotide sequence changes much more rapidly over time than nucleotide sequences coding for genes that are subject to selective forces. Although spacer DNA might not have a function that depends on its nucleotide sequence, it may have sequence-independent functions.Spacer DNA has practical applications that enable researchers and scientists to examine interactions between CRISPR proteins and bacteriophages.

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  1. magicmat

    Grouting half height kitchen splashback

    Hi everyone, I'm attempting to tile a new kitchen splashback using cube shape mosaic sheets and all going well, except I'm not sure how best to finish the grouting. The splashback will not go to the ceiling and there are no wall cupboards. There's several examples of the same style on this...
  2. W

    Spacer and adhesive

    Nearly there ... sorry for all of the banal questions. .... two more though! 1) I’m using a 250x400x7mm glossy tile. Do I use a 2mm or 3mm spacer? It’s a small en-suite room. 2) will this adhesive be ok? (see pic). The instructions say it’s good for 300x600 but I know that larger tiles may...
  3. G

    tile spacers for my new tiles

    Hello everyone, I am thinking of re-doing the tiles my living room. I've got 600x300 tiles and I'd like to know if it's acceptable to use 4mm tile spacers. I read some of the previous threads and noticed that most people prefer to use 3mm spacers for floor tiles, so I am starting to think that I...
  4. G

    Advice on re tiling kitchen backsplash

    Good afternoon everyone. I'm in the process of doing some refurbishment at my flat and the kitchen is my last area to tackle. I've taken.off the old tiles and that took the plaster with it. So a few weeks ago I had that re skimmed. The tiles I'll be using are 100x200 non bevelled metro tiles...
  5. L

    Kitchen Splash-back advice

    Hi all, I was hoping I could get a little advice. Currently doing a bit of tiling in the kitchen and have a few internal corners. When I have to cut a tile that reaches the corner do I need to replicate the same length of tile on the wall next to it or can I use a full length tile or do I use...
  6. C

    Metro tile spacers, 4mm. Is it going to look awful?

    hi, doing a diy job of filing our kitchen using metro tiles for b&q. We have started and have used the 4mm tile spacers but now we’ve realised that we probably should’ve used smaller. Is it going to look awful? Has anyone use 4mm before? We’ve chosen anthracite tiles and white grout. Should we...
  7. Beyond the blue

    Wickes spacer accuracy

    Being a retired engineer I always recon to have a good eye for measurements. so when I bought these spacers from wickes I had my doubts about their accuracy so stuck a couple in a pair of digital calipers. The 1mm spacer measured 1.72mm and 2mm spacer measured 2.42mm, so if you want a 2mm grout...
  8. M

    Full tile at top of splashback.

    Just about to start tiling the final wall in my kitchen I've tiled all the way around up to the units and now I'm just left with the final wall which doesn't have units. I'm setting out and to keep the level of the tiles from under the units on to the wall without units I would have to have a...
  9. G

    What size T spacers for metro style tiles (10cm x 20cm)

    Hi guys, I will be doing a kitchen splashback using metro tiles. The tiles are 10cm x 20cm in size. Should I go with 2mm spacers or 3mm spacers? Grout is Mapei Keracolor Super Fine (SF). Thank you. :)
  10. J

    Floor spacer sizes for wood effect porcelain tiles

    Hi, it's probably been asked before but here goes anyway. I believe the British standard recommends a minimum of 3mm spacers when laying floor tiles bit I'm looking at laying some 900x150mm wood effect porcelain tiles and was wondering if I can go less than 3mm? It's onto a good concrete base...
  11. Anthony Wills

    Substraight advice for splashback

    Ok, strange one. My customer was advised to chalk his walls. He has removed the old tiles and adhesive in the kitchen which, luckily, has left the walls in a fairly straight condition. But he had bought tubes of chalk and kind of applied it like you would with plaster to the whole wall. I have...
  12. T

    East Midlands Tiler needed for mosiacs kitchen splash back

    Job has been accepted Splashback*
  13. acaciaguy

    Setting Out Kitchen Splash back with metros - setting out for trim

    Hello all, I have a job this week doing a kitchen splash back and window reveal. The wall behind hob has a boxed in section which means extra cutting. I had originally planned and customer agreed that they wanted mitred externals. They have now changed their mind and want to use white round...
  14. T

    Kitchen splashback advice

    Hi folks, new to the forum and I'm a DIY novice with no tiling experience to speak of. Been reading through the forum recently and got some great tips and I'm considering a tiling course further down the line. Quick question for those with more experience; started tiling my kitchen splashback...
  15. T

    Which size tile spacer?

    I'm planning on installing coloured Bevel Metro tiles to kitchen splash back size 200/100 with a thickness of 7 mm. The manufacturer recommends a minimum of 2 mm for grout line, I'm not a fan of large grout lines and am hoping to either use 1 mm or 1 1/2 mm spacers. Has anybody had negative...
  16. G

    Help needed with mosaic splashback

    Hello looking for a bit of advice. I’m planning on doing a splash back above my sink. So far I have the tiles and trim. Was wondering what’s the best adhesive and grout to use as there is glass tiles on the mosaic sheet and I’ve also heard that the tiles need treated with something after the...
  17. P

    900x150 Porcelain Planks Spacer Size

    Hello All, Looking for a little bit of advice for an upcoming job... 900x150 porca planks - what spacer size would you use? 3mm, 5mm or other? Customer wanted them laid in half brick bond but have advised should go for 3/4 running bond to help avoid bowwing/lippage issues. Don't see too many...
  18. F

    Tile Levelling Spacers (Again)

    I know from reading old posts on here this can be a sensitive subject - but I have some observations. I have used tile lash on a brick bond kitchen floor with 600 x300 and thought it was a fantastic product but I realised I was struggling because of the shape of the base bends up and you have...
  19. L

    Seal Slate backsplash behind stove

    We have just had a slate backsplash installed all round the kitchen benchtops. The builder sealed it with 2 coats of Tasmanian Chemicals Premium Long Life Gloss, which the slate supplier had sold us for that specific job. Unfortunately, steam from the kettle left a plume of white glaze across...
  20. C

    recomendations for tile levelling spacers

    hello all, i'm just about to start tiling my bathroom and thinking to use those levelling spacers, are they recommended ? i have some 300*200 *8mm ceramic tiles. i have an ardex sealing kit for around bath. guess i'll get ardex adhesive ? the walls aren't very flat , a mix match of...
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