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primer

A primer is a short single-stranded nucleic acid used by all living organisms in the initiation of DNA synthesis. DNA polymerase (responsible for DNA replication) enzymes are only capable of adding nucleotides to the 3’-end of an existing nucleic acid, requiring a primer be bound to the template before DNA polymerase can begin a complementary strand. Living organisms use solely RNA primers, while laboratory techniques in biochemistry and molecular biology that require in vitro DNA synthesis (such as DNA sequencing and polymerase chain reaction) usually use DNA primers, since they are more temperature stable.

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  1. SanDiegoChip

    USA Questions About Tile Over Tile Plan for Walk-in Shower

    Hello, Just joined the forum and am hoping to get some advice on a project. I live in San Diego, CA. My wife and I have been unhappy with our master bath walk-in shower floor since we bought the house 25 years ago. We do not like the aesthetics and the floor always looks dirty. For the last 10...
  2. A

    Bath Shower wall prep after tiles fell off

    My bathroom was tiled 5 years ago with 60x30 tiles which became loose and came clean off with a little wiggle. The installation was prior to when I moved in. The walls look sound and flat and seemed to have been newly plastered before the tiling was done. My question is what do I need to seal...
  3. DrBrown

    Can I use Rendagrip as a primer

    I am going to tile over a small tiled floor at my own property. I do intend to prime the tiles first with Mapei Eco Grip Plus however I was just wondering if I could use Ever Build Rendagrip as I have plenty of the stuff.?
  4. R

    Preparation Primer for tiling over sound/solid floor tiles

    Hi, I'm wanting to tile over kitchen floor tiles, tiles are soundly stuck down. Main issue is they're smooth and shiny. Cannot be arsed to grind them. What's the best primer before laying over them. Thanks in advance
  5. M

    Primer G and masonry/brick wall

    Hello I've trawled forums and read the Mapei Primer G packaging etc but not sure I've got 100% comfort on this one and hopefully it's basic... Is this product suitable for bare brick/masonry walls? Most documentation seems to refer to plaster/plywood/screeds etc so just want to be clear...
  6. D

    I forgot to use primer before tanking

    Hi. I have a new build house which i am fitting out 2 shower areas. I started putting on the tanking kit and membrane but i forgot to prime the walls first. Is this a problem? One shower area is a hard coat of plaster on concrete block and the other area is plaster and cement board. Am I OK...
  7. C

    Primer for new skim under waterproofing

    Hiya, just had a long discussion at Topps Tiles about which primer would be best to use under the waterproofing liquid. The technical sheet doesn't rule out to use the liquid on new skim (on bathroom plasterboard), provided it is primed first, but doesn't specify a primer. The store staff didn't...
  8. H

    What primer for backer board?

    …for going under flex adhesive to bed hardiebacker boards on 1) t&g chipboard floor and 2) brick wall Is waterproof pva adequate?
  9. A

    Priming and tanking - various surfaces

    Hi all - we’re doing a bathroom refit and have some questions on best practices for tanking (we’ve bought the Mapei Aquadefense product, but the instructions are as clear as mud): (1) we’ve got plywood floor, onto which vinyl will be laid. Does this need tanking? If so, should it be primed...
  10. N

    Preparation Anhydrite screed prep

    Hi guys technical question hoping maybe you have an answer as I have never came across this before we have an anhydrite screed floor we sanded it hoovered then sealed twice came In Today and the sealer looks like it’s peeling in certain areas have u ever had this the floor is completely dry...
  11. Alf_Laden

    Tiling primer help needed!

    Hi all, Doing my first DIY bathroom tiling job, just a 2x3m space, but have run into an issue with primer... One of the walls is an external solid wall (Victorian property) and this wall was rendered, then skimmed with gypsum plaster (months ago). I heard that I needed to prime this wall...
  12. S

    Diluting primer before tiling

    Am getting conflicting answers online and manufacturer support lines, have a bathroom which has Hardiebacker, moisture plasterboard and multi finish on different walls and is being fully tiled. Can anyone advise correct dilution ratios for priming these walls with Mapei Primer G? Will be using...
  13. T

    Acrylic primer didn't bond to Larsen Tile Adhesive

    I had an issue recently that I've never seen before and any help or advice would be greatly appreciated. I used a Larsen acrylic primer on gyspum walls as instructed on product (walls had been skimmed 8+ weeks previous and moisture tested prior to sealing and tiling). 18 months later and it...
  14. N

    SBR latex based primer for no more ply

    need some advice, did not read instructions properly, and put undiluted SBR tilers primer onto backer boards , the boards have a shiny finish to them now -- will this be an issue when tiling ?
  15. M

    Massive Help Needed with old Plaster and Primer (Please, I'm begging someone)

    Hello All, I would just like to start off by saying that I've been in the construction industry for roughly 7 years as a Cladding Site Manager and Facade Engineer, but apologetically, I have zero expertise in Tiling and I am attempting a DIY Re-Tiling job on a very small budget for my...
  16. M

    My 1st Job Using Blue Grit and Mapei Plaster Primer

    Hello All, I would just like to start off by saying that I've been in the construction industry for roughly 7 years as a Cladding Site Manager and Facade Engineer, but apologetically, I have zero expertise in Tiling and I am attempting a DIY Re-Tiling job on a very small budget for my...
  17. E

    Anhydrite+Ditra+Porcelain - Mapei Primer or not / Keraquick with or without Latex Plus

    Hello Everyone! First time writing here. 😅Contemplating the horrendous prospect of installing underfloor heating in a fully inhabited house with furniture and belongings now in the garden I have read a ton of posts on this forum and you’ve been a marvellous help. So, a zillion thanks ahead of...
  18. B

    plywood primer

    What's your tiling question? Pictures can be added.
  19. A

    Bal APD primer not doing its job on Marmox and Impey waterguard

    My first big tiling job. It's a wet room and I'm at the stage where I have Marmox boards on floor and walls and an Impey former. I'm just about to put down the Impey Waterguard membrane and the instructions specify priming with the supplied Bal APD primer. I tested the BAL primer inadvertently...
  20. B

    SBR primer in bathroom before tiling?

    Should I prime bare plaster in the bathroom with SBR before tiling? I've heard it will help with the water resistance and help the tile adhesion. The tile sizes are 600x300. Any help would be appreciated, Cheers Brian.
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