Morning steve. It’s now not advisable to tile too plywood now , a change to bs5385 around 3 yrs ago i think now. Too much inferior Chinese plywood lol.Substrate problem. If your floor is wooden floorboards I wouldn't tile the floor unless it's been cross lined with not less than 10mm ply screwed (not nailed) down with screws every 15cm minimum. Boards beneath need to be thoroughly fixed on the rafters too. Anything less the adhesive will crumble away due to deflection issues (as above) and the tiles will crack. In short...wood is flexible and tiles are not so you need to make the wood thick and strong to stop the bending. This will make your bathroom floor 20 to 25mm higher than the board level and many people don't like that.