primer

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A primer is a short single-stranded nucleic acid used by all living organisms in the initiation of DNA synthesis. DNA polymerase (responsible for DNA replication) enzymes are only capable of adding nucleotides to the 3’-end of an existing nucleic acid, requiring a primer be bound to the template before DNA polymerase can begin a complementary strand. Living organisms use solely RNA primers, while laboratory techniques in biochemistry and molecular biology that require in vitro DNA synthesis (such as DNA sequencing and polymerase chain reaction) usually use DNA primers, since they are more temperature stable.

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  1. kiteboy

    What primer and adhesive - underfloor calcium sulphate

    Hi all Just wondering if there are any recommendations for correct primer and adhesive for a kitchen with underfloor (piped) heating on a calcium sulphate screed Ive was planning on using Bal APD primer and BAL adhesive but Ive just read that BAL stuff is cement based so perhaps no good...
  2. S

    Primer after tanking Hardie Backer?

    I’ve got Hardiebackers for my en suite walls and will be tanking them with BAL WP1. Do I then need to apply a primer for the adhesive after tanking? I’ll be using Mapei Keraflex S1 as my adhesive. Cheers.
  3. D

    SBR or PRIMER G for SAND/CEMENT RENDER??

    Sorry if this thread has been posted but i really can not find the answer. I want to know which primer to use on sand/cement render prior to tiling, i have mapei adhesive, so maybe primer g? or SBR (as its on sand/cement render)? Do i need to dilute? I have also use Mapegum WPS tanking to the...
  4. J

    Mapei Primer G. Question

    How soon after painting plaster with Mapei primer G can you begin tiling? I can't find an answer in their blurb TA
  5. S

    Primer & P5 moisture resistant T&G chipboard before I laying Aquapanel?

    Hello, please can someone advise me before I mess up and waste loads of time and money! Do I need to prime P5 moisture resistant T&G chipboard before I lay Aquapanel? Also, do I have to then prime the Aquapanel before I lay the ceramic floor tiles? I will be using Mapei flexi quick set tile...
  6. C

    Applying primer methods

    Hi guys How does everyone apply primer do you use a old sponge and sponge it on or do you use a large paint brush? Cheers in advance
  7. E

    Right adhesive and primer?

    Hi. Just wanted to make sure I’m doing this right... I have moisture resistant plasterboard dot and dabbed on walls. Floors have tile backer board down. Walls to be tiled top to bottom, floor tiles too. All tiles are 600x300. I am looking at getting the adhesive and primer. Was going to...
  8. A

    bostik primer coming off when scrapping feet on

    Have used bostik primer yesterday and it took some hours(4) to dry to walk on without anything coming off. I was going to put my self leveler on top however when i twist my feet on it firmly, some comes off. Would this be the norm or has this not bonded properly. I was of the thinking that this...
  9. M

    My 1st Job Levelled with addy - Is primer needed?

    I've boarded my bathroom with moisture resistant plasterboards and hardie tilebacker for the shower area. I've then primed the boards with weber pr360. Then used scrim cloth to cover the joints and used weber.set SPF addy to skim over the joints and level the plasterboard tapers and any other...
    • Mark Parmley
    • Thread
    • needed primer
    • Replies: 2
    • Forum: DIY Tiling Forum
  10. G

    BAL Can I use BAL ADP primer with Mapei Kereflex on plaster board?

    Shortly I hope to tile a wall ( skimed plaster board ) that has previously been painted with matt emulsion. The plan is to sand / grind off the paint, then prime and tile. The tile wt.is within the loading capacity. Questions. Is the wall prep ok? Can I use BAL ADP primer with Mapei adhesive...
    • Geoff Stafford
    • Thread
    • adhesive bal bal adhesive board building adhesives can grout mapei mapei adhesive mapei grout plaster plaster board primer
    • Replies: 1
    • Forum: DIY Tiling Forum
  11. L

    Which primer do you like for concrete floors before S/L?

    I have 3-4 choices from the "builders depot " . wondering if most are roughly the same or one stands out as been really "better " want to level up with self levelling after
    • LJD
    • Thread
    • canada concrete floor floor tiling floors primer which
    • Replies: 3
    • Forum: Canada Tile Advice
  12. S

    Any primer required?

    Just about to start tiling an older concrete floor (20 ish years old) that has previously had self levelling applied with carpet on top. Is in sound condition with a few minor hollows, - nothing major. Going to lay 9mm porcelain tiles (600 x 600) and wondering if any form of primer required...
    • Stuartpaul
    • Thread
    • primer required
    • Replies: 2
    • Forum: DIY Tiling Forum
  13. R

    Tiling over tiles primer

    hi all I wondered what people recommend as a primer before tiling over old tiles? Any suggestions greatfully received. Thanks
    • Rob Harrison
    • Thread
    • canada over primer tile tiles tiling
    • Replies: 9
    • Forum: Canada Tile Advice
  14. B

    BAL Bal apd primer used with Mapei powder addy is this ok ?

    Can I mix match these brands
    • Ben Ross
    • Thread
    • adhesive bal bal adhesive building adhesives grout mapei mapei adhesive mapei grout primer this used
    • Replies: 1
    • Forum: Tile Adhesive / Grout Advice
  15. M

    Tiling on fresh plaster

    Had the bathroom skimmed over 2 weeks ago and is now dry. Some chases in the wall had to be bonded and skimmed and its still a little wet but I want to get started on the walls that are dry. Bal adhesive pack im using says to prime with bal prime APD before, is there an alternative brand people...
  16. J

    Type of Primer

    i am about to tile my bathroom using a rapid set (powdered) adhesive using 500 x 200mm ceramic tiles. One of the walls has previously been painted. The paint is well adhered to the surface cant scrape off. What advice on a good primer to use on this surface Thanks
  17. V

    Mapei tanking peeling of the wall

    Hi Ive tanked bathroom walls (aquapanel and hardie) using Mapei aquadefense(no primer as advised by mapei support) After 2 days product peeling of the wall in large sheets. Ive rang Mapei again to hear that is perfectly normal And "tiles won't come off as different type of pressure is...
    • V Man
    • Thread
    • adhesive aquapanel grout mapei mapei adhesive mapei grout mapei tanking primer tank tanking wall waterproofing wetroom
    • Replies: 15
    • Forum: Tanking and Wetrooms
  18. A

    Primer evostik SBR?

    I'm using no more ply however there is no SBR primer in the tile shop so they gave me evostik. Is the 918 evolved primer the same stuff as the SBR recommended for the ply. There's just something at the back of my mind telling they are the same primer. I forget where I heard this not being the...
  19. A

    What acrylic primer?

    Happy new year gents. I'll soon be fixing very large tiles on water resistant plasterboard, is the Mapei acrylic primer from Screwfix okay or what would you recommend? Regards
  20. J

    T&G boards tiling on top of????

    hello, ive got a job coming up and there could be a deal with the step at the entrance of the wet room. ive looked in tilemaster product guide and it says you can tile on top t&g boards after priming with prime+grip primer. Has anyone done this before??
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